The mutan containing α-1,3 bond is an insoluble portion of glucan which is the main component of dental plaque. The secretion of mutanase was assessed with mutan-digesting Streptomyces isolated from soil and the factors affecting its activity
was
studied, obtaining the following result.
Mutan-digestings Streptomyces was identified as Streptomyces exfoliatus by its characteristics. The effect of dextranase was identified on the media containing blue dextran. A clear zone was produced by Streptomyces exfoliatus on the media
containing
blue mutan, so showing the secretion of mutanase. A clear zone was significantly produced on the media overlayed with agar containing blue mutan. A clear zone was produced at 2 days after the inoculation of Streptomyces exfoliatus on the media
containing below a concentration of 0.025% glucose, at 3 days on the media containing 0.05% glucose, and at 4 days on the media containing 0.1% glucose. Mutan-digestion wasn't appeared early by adding other carbohydrates.
The higher concentration of peptone, the later appearance of clear zone was on the midia containing below a concentration of 0.1% peptone.
These results indicated that the secretion of mutanase was identified from mutan-digesting streptomyces on the media containing blue mutan, and a clear zone was appared lately on the media containing higher amount of glucose.
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